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Reference : UMR5535-VALDAR-003
Workplace : MONTPELLIER
Date of publication : Thursday, September 23, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 December 2021
Proportion of work : Full time
Remuneration : About 2690 € brut/month
Desired level of education : PhD
Experience required : 1 to 4 years
The recent findings that changes in metabolic programs condition T cell activation and differentiation as well as susceptibility to HIV-1 infection have opened new strategic avenues for controlling disease progression. While the new energetic and biosynthetic demands of an activated T lymphocyte can be met by different nutrients, several elegant studies have shown that the phenotype and fate of the cell are conditioned by the pathways via which a given nutrient is utilized. In the context of HIV-1 infection, our group and others have shown that HIV-1 susceptibility is governed by the T cell's metabolic activity, revealing the importance of oxidative phosphorylation (OXPHOS) in infection. Specifically, our studies highlighted the differential contribution of specific nutrients (glucose and glutamine) as critical determinants in HIV-1 infection. Furthermore, in the context of this study, both glucose and glutamine transporters were highly upregulated, and we therefore propose to evaluate the impact of the crosstalk between several nutrient transporters, as a function of extracellular nutrient availability, on HIV-1 infection in different CD4 T cell subsets.
The selected candidate will contribute to all aspects of the proposed project using multi-levels, interdisciplinary approaches.
The selected candidate will report/communicate regularly her/his results
The candidate must hold a Ph.D in biology with <2 years post-doctoral experience.
The candidate should have a strong background in immunology/virology and expertise in several of the following areas will be appreciated: human primary tissue culture, virology (virus production and titration assays), molecular and cell biology techniques such as quantitative PCR, viral transduction and shRNA, flow cytometry analyses.
Experience in single cell RNAseq and/or metabolic analysis with an interest in bio-informatics will be a plus.
The candidate will be highly motivated, have communication skills, team effort spirit, organization skills and have independent thinking.
The applicant will join the dynamic “Hematopoiesis and Immunotherapy” group (https://www.igmm.cnrs.fr/team/hematopoiese-et-immunotherapie/) at the - Institut de Génétique Moléculaire de Montpellier (IGMM), Montpellier, France directed by N.Taylor/ V. Zimmermann and under the supervision of V. Dardalhon/ C. Mongellaz. The IGMM is a multidisciplinary institute with about 220 people (27 nationalities represented) divided into 16 research groups. The campus provides access to state-of-art scientific and technologic facilities including genomics, imaging, flow cytometry. The project will also benefit from well-established collaborations in France but also in the US.
Constraints and risks
- The candidate will work on human primary cells in L2/A2 facilities
- Possible variability of working hours
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