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Reference : UMR5292-SYLMAZ-001
Workplace : BRON
Date of publication : Tuesday, December 04, 2018
Type of Contract : FTC Scientist
Contract Period : 36 months
Expected date of employment : 1 January 2019
Proportion of work : Full time
Remuneration : 2555 € gross salary per month
Desired level of education : PhD
Experience required : 1 to 4 years
The team "GEnetics of NeuroDEVelopment" (GENDEV) is seeking a highly motivated post-doctoral fellow for a fully funded 3-years position to work on a project aiming to unravel the role played by minor splicing in embryonic development through the study of zebrafish models of a family of neurodevelopment disorders.
Biallelic mutations in the non-coding gene RNU4ATAC were identified by our group in the Taybi-Linder syndrome (TALS, OMIM 210710, Edery P et al. 2011 Science). TALS is a rare autosomal recessive syndrome characterized by severe brain malformations. RNU4ATAC is transcribed into U4atac snRNA, a component of the minor (also called U12) spliceosome, a ribonucleoproteic complex that removes a restricted subset (<1%) of introns during the mRNA maturation process. Recently, two other very rare congenital disorders have also been attributed to biallelic RNU4ATAC mutations. We combine multiple approaches including genetic epidemiology, clinical genetics, genomics, transcriptomics, bioinformatics, molecular biology, cell biology and zebrafish models to decipher the molecular causes and physiopathology of RNU4ATAC-associated syndromes to improve knowledge on the role of minor (U12) intron splicing in brain development and to allow accurate information, genetic counselling and medical care of patients with a disorder of brain development.
The candidate will need to:
-perform in zebrafish morpholino-driven and CRISPR gene editing;
-conduct development and morphology analyses on these zebrafish models, especially focused on brain;
-perform molecular and cellular biology techniques, including transcriptional profiling via RNA-seq together with our bioinformatician collaborators;
- analyse and communicate the produced data;
- write the publications.
Candidates must hold a PhD degree in Genetics, Neurosciences, Biochemistry, Cell Biology or other related fields, and a postdoctoral experience of less than 3 years. Excellent skills in molecular and cell biology techniques and in zebrafish model are required; Experience in neuroscience would be a bonus.
The GENDEV research team was created in 2016 at the Lyon Neuroscience Research Centre (CRNL) and is composed of clinical and molecular geneticists. The CRNL is a neuroscience research centre located within new buildings next to four large hospitals that integrates the multidisciplinary expertise of 380 members (14 teams) with a synergistic approach of brain organization and development, cognitive functions and mental states in physiological and pathological conditions, using a broad spectrum of research tools ranging from cognition to genetics. The position will provide opportunities for a young researcher to benefit from the GENDEV collective first-rate expertise on human syndromes, genomics, transcriptomics, bioinformatics, biochemistry and zebrafish model to perform a multidisciplinary research study.
The contract is financed by the ANR (National Research Agency). Applications must be submitted on emploi.cnrs.fr site; they should include a CV and a letter of motivation summarizing the scientific interests of the candidate, as well as contact details for two referees.
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