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Reference : UMR5239-ISASER-008
Workplace : LYON 07
Date of publication : Tuesday, November 05, 2019
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 January 2020
Proportion of work : Full time
Remuneration : Approx. 3 000€ monthly gross
Desired level of education : PhD
Experience required : 1 to 4 years
The applicant is expected to work independently on questions relating to the mechanism of homologous recombination and the spatial organization of the genome in the model organism S. cerevisiae. He/She will formulate hypothesis, design and perform experiments, and analyze the data he/she will generate.
-Hypothesis formulation and experimental design
-Classic molecular genetics (cloning, yeast strains construction, etc)
-High-throughput genomic techniques
-Dissemination of the research output
Candidates should be highly motivated and be willing to take on intellectual, scientific and technical challenges. Preference will be given to candidates with less than three years of experience after his/her Ph.D. the following qualities are expected:
-Expertise in molecular genetics.
-Expertise in the field of DNA replication, repair and recombination
-Experience with yeast genetics.
-Computational skills are not required, but a plus.
The laboratory will start on January 1st 2020 at the fully renovated Laboratory of Biology and Modelling of the Cell (LBMC) in the privileged environment of the Ecole Normale Superieure (ENS) campus in Lyon. The work will be performed in collaboration with the laboratory of Romain Koszul at the Institut Pasteur, and involved being detached there. It is supported by a generous ERC grant.
The project aims at tackling the as-yet mysterious mechanism of homology search that takes place during the repair of DNA double-strand break (DSB) by homologous recombination. This process involves events occurring at different scales, from DNA sampling by a specialized nucleoprotein filament assembled at the break site, to the spatial reorganization and increased mobility of chromatin upon activation of the DNA damage checkpoint. The goal of this project is to achieve an integrated and quantitative framework of homology search in cells, by using a cycle between experiments in S. cerevisiae and computer simulations. The experimental systems and methodologies are readily available in the laboratory (including novel assays that enable to track early recombination intermediates in cells) and ample preliminary results have been gathered making this project immediately fun, and low risk/high gain.
Constraints and risks
The recruitment will be made on an ERC grant managed by the CNRS (DR7 – Rhone-Alpes). However a significative part of the research project will take place in UMR3525 (Institut Pasteur – Paris).
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