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Portail > Offres > Offre UMR5203-GUILEB-003 - CDD Chercheur, Dynamique structurale des récepteurs couplés aux protéines G (H/F)

Post-doctoral position in membrane protein biochemistry and structural biology

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mercredi 10 mars 2021

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General information

Reference : UMR5203-GUILEB-003
Workplace : MONTPELLIER
Date of publication : Wednesday, February 17, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 April 2021
Proportion of work : Full time
Remuneration : between 2400 and 2700 € growth wage per month
Desired level of education : PhD
Experience required : 1 to 4 years

Missions

The selected candidate will developed a research project that aim at deciphering the activation mechanism of G protein coupled receptors (GPCRs). The candidate will express and purify GPCR and GPCRs signalling complexes and performed biophysical and structural characterisation of the purified receptor. The main objective is to decipher the structural dynamics of GPCR at the atomic scale.

Activities

-Molecular biology and protein engineering (mutagenesis, unnatural amino acid labelling)
-Membrane protein expression and purification
-sample reconstitution in membrane-mimicking environment (nanodisc)
-Biochemical and pharmacological characterisation of purified receptors.
-Crystallisation in lipidic cubic phase
-Results analysis.

Skills

- Molecular Biology
- Cell culture,
- Large scale expression and purification of membrane proteins.
- Membrane protein biochemistry.
- GPCRs crystallisation
- synchrotron data collection
- Model building

Work Context

The IGF is University, CNRS and INSERM research unit located on the Medical Sciences campus in Montpellier (http://www.igf.cnrs.fr). IGF 19 research teams develop multidisciplinary research project from the atomic scale to animal model. Our institute is internationally established in the field of GPCRs and molecular pharmacology and provide a state of the art research environment.

The selected candidate will be part of the team Molecular and structural mechanism of neuromodulation, in the neuroscience department.

We are seeking a highly motivated biochemist with experience in molecular biology, membrane protein expression (insect and mammalian cells) and purification. Experience with membrane protein cystallisation would also be appreciated but not essential, as training will be provided.

For application, a letter of motivation, a CV and the names of referees should be sent to Guillaume Lebon: guillaume.lebon@igf.cnrs.fr

Constraints and risks

n/a

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