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Reference : UMR5203-ANAMEN-001
Workplace : MONTPELLIER
Date of publication : Friday, November 12, 2021
Type of Contract : FTC Scientist
Contract Period : 30 months
Expected date of employment : 1 April 2022
Proportion of work : Full time
Remuneration : starting salary at 33k/year before tax - based on the CNRS pay scale
Desired level of education : PhD
Experience required : Indifferent
The recruited candidate will be working on solving structures of GPCR complexes using single particle cryoEM and investigating their interactions with lipids.
- Perform GPCR expression and purification and all functional experiments.
- Perform cryoEM grid preparation, data collection and analysis for structure resolution.
- Keep up-to-date lab books, organize and store data according to lab rules.
- Participate in lab meeting, general lab organization and up-keep.
- Write up results for publication and presentation at meetings/conferences.
- A PhD, or about to be awarded, in biochemistry, biophysics or structural biology with relevant associated publication(s).
- Experience with membrane proteins expression and purification is a must. Specifically, experience in eukaryotic expression system (mammalian cells and/or insect cells) will be greatly appreciated.
- Experience in cryoEM is desirable.
- Previous experience working with GPCRs will be appreciated but isn't essential.
- The ability to work collaboratively and be a team player is essential. The candidate should also be able to work independently and efficiently.
- Good oral and written skills in English is also essential as all lab business and meetings are conducted in English. French speaking is not required.
Our team is made up of 10+ researchers, postdocs, engineers and PhD students of different nationalities forming a friendly and dynamic group. Our work mainly focuses on the study of Class C GPCRs using innovative pharmacological tools such as nanobodies and photoactivable ligands, as well as FRET to analyze GPCRs function and conformations at the molecular and single molecule level (collaboration with E Margeat, on campus). The advertised position is funded by an ANR grant coordinated by Dr Anaïs Menny, who will be your direct supervisor, dedicated to expanding our research themes to single particle cryoEM and other biophysical approaches.
For the realization of the project, you will have access to an EM facility housing a 200 KeV JEOL with FEG, K3 camera and GIF, and a 120 keV JEOL for screenings. Your work will also be supported by several high-end platforms covering GPCR functional assays and light microscopy.
The scientific environment at the institute provides ample interaction possibilities and support from other teams studying GPCRs, ranging from neuropharmacology to cryoEM and crystallography.
Please provide a CV, a short cover letter explaining your research interests, and contact details of two references
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