En poursuivant votre navigation sur ce site, vous acceptez le dépôt de cookies dans votre navigateur. (En savoir plus)
Portail > Offres > Offre UMR5168-CHLZUB-003 - Chercheur(se) postdoctoral(e) en biologie structurale et biophysique H/F

Postdoctoral Fellow (M/F) in structural biology or biophysics

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mercredi 25 mai 2022

Assurez-vous que votre profil candidat soit correctement renseigné avant de postuler. Les informations de votre profil complètent celles associées à chaque candidature. Afin d’augmenter votre visibilité sur notre Portail Emploi et ainsi permettre aux recruteurs de consulter votre profil candidat, vous avez la possibilité de déposer votre CV dans notre CVThèque en un clic !

General information

Reference : UMR5168-CHLZUB-003
Workplace : GRENOBLE
Date of publication : Wednesday, May 4, 2022
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 September 2022
Proportion of work : Full time
Remuneration : pay is between 2663.79 and 3953.59 € euros gross per month depending on experience
Desired level of education : PhD
Experience required : 5 to 10 years

Missions

The candidate will be part of the Integrated Structural Biology and Plant Development team of the Laboratoire de Physiologie Cellulaire et Végétale in Grenoble. We have identified a protein important for temperature sensing in Arabidopsis called EARLY FLOWERING 3 (ELF3), a core component of the circadian clock. This protein contains an intrinsically disordered region and a prion-like domain that undergoes liquid-liquid phase separation (LLPS) in vitro and in vivo. The goal of the project is to determine the underlying mechanisms important for phase separation in vitro and in vivo and to determine the physiological significance of LLPS in planta. The candidate will be responsible for conducting small angle x-ray scattering (SAXS), AFM and fluorescence microscopy experiments for structural characterization of the protein as a function of different conditions in vitro. The candidate will perform transgenic experiments using the model plant, Arabidopsis thaliana, and characterize the formation of ELF3-containing puncta using FRAP and FRET-FLIM. In addition, the candidate will aid in supervising students/technicians. The candidate will be located and perform his/her experiments at the CEA in Grenoble. The candidate will be supervised by C. Zubieta.

Activities

The candidate will develop protocols for the expression and purification of ELF3 full length and truncation constructs. This requires recombinant expression tests in prokaryotic and eukaryotic expression systems and familiarity with these techniques are required. The candidate will perform SAXS data collection and structure solution at the ESRF (France) and Diamond Light Source (UK). In addition, different biochemical and biophysical techniques including AFM, EM, optical and fluorescence microscopy may be used to characterize the structure of the protein of interest as a function of temperature. The candidate will help supervise thesis and Master's students, contribute to the redaction of manuscripts and present results at national and international conferences. The results obtained will contribute to our understanding of plant thermosensing and response. These results will be basic research but with potential long-term applications to the field of bioengineering of desired traits in crop species.

Skills

Required skills include experience in plant biology, molecular biology (design of different constructs, expression vectors), protein biochemistry (protein expression, purification and characterization), fluorescence microscopy (FRAP) and SAXS. The candidate will have a doctorate in plant biology, structural biology or a related field. The candidate must be fluent in spoken and written English and ideally possess a working knowledge of French. Candidates must have a demonstrated publication record in the field. Candidates must be able to work collaboratively in a team.

Work Context

The project will take place at the Laboratoire de Physiologie Cellulaire et Végétale, located on the CEA campus in Grenoble. The LPCV (https://www.lpcv.fr), part of the Institute de Recherche Interdisciplinaire de Grenoble (IRIG, https://www.cea.fr/drf/IRIG/Pages/Presentation.aspx), consists of ten research teams and over 200 researchers in plant and cell physiology. The LPCV is a diverse laboratory specializing in plant developmental biology, structural biology, microscopy and biochemistry and includes researchers from the CNRS (http://www.cnrs.fr/index.php), CEA, INRAE and the Université Grenoble Alpes. The project also implicates the structural biology teams of the European Synchrotron Radiation Facility (ESRF https://www.esrf.fr). This close collaboration allows the candidate access to the small angle X-ray scattering beamline (BM29) of the ESRF.

Grenoble is a dynamic city with a vibrant international research community located in the Alps. In addition to its scientific appeal, Grenoble offers mountain sports including rock climbing, VTT, road cycling and skiing.

Constraints and risks

Travel to the UK and Germany for data collection may be required (international travel).

We talk about it on Twitter!