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Portail > Offres > Offre UMR5086-MATGUZ-001 - Assistant ingénieur(e) en microbiologie moléculaire (H/F)

Assistant Ingenieur Position in Molecular Microbiology

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : lundi 13 décembre 2021

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General information

Reference : UMR5086-MATGUZ-001
Workplace : LYON 07
Date of publication : Monday, November 22, 2021
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 1 February 2022
Proportion of work : Full time
Remuneration : 1967
Desired level of education : 2-year university degree
Experience required : Indifferent

Missions

- get bacteriology and molecular biology experiments up and running in the lab
- participate in setting up the lab (ordering, logistic…)
- perform molecular biology experiments and building bacterial strains
- participate to the lab projects to explore regulations in Caulobacter cell-cycle at the single cell level (microscopy, flow cytometry,…)

Activities

- building bacterial strains of Caulobacter crescentus (preparation of competent cells, transformations, genotype verification)
- building plasmids (primer design, cloning, analysis of sequencing results).
- bacterial growth, phenotypic tests
- participate in setting up flow cytometry experiments, western blots, bacterial two-hybrid,…
- manage lab stocks and ordering
- analyse and restitue results
- opportunity to participate to the diffusion and valorization of lab results (oral presentation, publications,…)

Skills

- molecular biology : PCR, cloning, analysis of sequencing results
- bacteriology and bacterial genetics
- strong organizational and team work abilities
- knowledge of other technics like western blot, flow cytometry, microscopy is a plus
- good level in written and spoken English is a plus

Work Context

You will be part of a newly formed team interested in coupling mechanisms between DNA replication and asymmetric cell division in the bacterium Caulobacter crescentus as well as regulations that control the transition towards a proliferation state during the cell-cycle. The lab combines approaches in bacterial genetics and microbiology, transcriptional and protein quantifications at the population level during the cell-cycle and fluorescence microscopy on living cells at the single cell level.

Additional Information

1-year position with a possible 2-year extension

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