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Portail > Offres > Offre UMR5075-JERGAU-015 - Ingénieur.e de recherche en microbiologie / biologie cellulaire affecté.e au groupe PNEUMOCOQUE de l'IBS (H/F)

Research engineer in microbiology/cell biology assigned to the Pneumococcal Group of the IBS (H/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : jeudi 28 octobre 2021

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General information

Reference : UMR5075-JERGAU-015
Workplace : GRENOBLE
Date of publication : Thursday, October 7, 2021
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : According to experience and from €2,462 gross
Desired level of education : PhD
Experience required : Indifferent

Missions

In the framework of its research activities, the Pneumococcus Group of the Institute of Structural Biology is recruiting a research engineer for a 12-month renewable period. In addition to participating in the life of the laboratory, the main activity of the person recruited will be to implement and carry out experiments related to the ANR project "PneumoClick". The objective of this project is to propose a new therapeutic solution specifically targeted against the human pathogen Streptococcus pneumoniae in order to inhibit its growth while preserving the microbiota and slowing down the appearance of antibiotic resistance. To this end, we are exploiting the innovation of click chemistry and the specificities of the biosynthetic pathways of the S. pneumoniae cell wall. Our main objective is to demonstrate that it is possible to coordinate the chemical reactivity of new molecules with the biosynthetic processes of teichoic acids (TA) and peptidoglycan (PG), the two main components of the bacterial cell wall, in order to spatio-temporally control the formation of new artificial covalent bonds in the cell wall in vivo. We will test the ability of this innovative strategy to selectively inhibit the growth of susceptible and resistant strains of S. pneumoniae.

Activities

You will carry out research work from setting up the experiment to writing up the results and protocols:
1/ You will carry out complex scientific experiments in a reliable and reproducible manner within the given deadlines:
- Carry out bacterial cultures and growth monitoring
- Test the molecules developed by our chemist partner (incorporation into the bacteria, harmlessness on bacteria and mammalian cells)
- Carry out labelling and cross-linking of peptidoglycan and teichoic acids on the bacterial surface by click chemistry
- Routine culture of mammalian cells
- Acquire data by epifluorescence microscopy and cytometry

2/ You will document and process the experimental procedures:
- Analyse, interpret and format data
- Present the results clearly and accurately, including in English
- Be a force for proposal for the development and improvement of experiments

3/ You participate in the life of the laboratory
- Maintain the laboratory (monitor reagent stocks, tidy up the dishes, organise the areas, manage waste, etc.)
- Prepare solutions for common use
- Assist in the technical supervision of trainees
- Participate in laboratory meetings and scientific seminars of the Institute

Skills

You have a PhD in microbiology with good knowledge in cell biology and cell culture
1) Basic knowledge
- In-depth knowledge of basic microbiology, molecular mechanisms of bacterial division, cell wall composition
- General knowledge of cell biology
- Basic knowledge of chemistry would be a plus
- English language: B2 preferred (according to the Common European Framework of Reference for Languages)

2) Technical skills
- Fluorescence microscopy and flow cytometry techniques
- Knowledge of mammalian cell maintenance (culture, passage, counting, freezing)
- Knowledge of data processing and computer science applied to image analysis
- Knowledge of and compliance with health and safety regulations in force in laboratories, particularly in the P2 laboratory

3) Behavioural and relational skills
- You have a great capacity for autonomy and are able to make proposals
- You have an open mind and are able to listen
- You have good interpersonal skills and an ability to integrate and adapt to the team
- You are organised and conscientious
- You are able to communicate and report on your work
- You are creative and have a spirit of initiative

Work Context

The IBS (300 people) is a joint CEA-CNRS-UGA research unit inserted in the scientific environment of the Grenoble peninsula, which hosts CEA and CNRS laboratories, but also the European structures of the ILL, ESRF and EMBL, creating a dynamic local synergy concretized by strong partnerships (Partnership for Structural Biology - PSB).

Under the supervision of the ANR project leader and the authority of the group leader, you will work in the Pneumococcus Group, which is composed of 3 researchers, 1 teacher-researcher, 3 technical experts and two doctoral students, plus trainees at L3 to M2 level. Specific training may be offered when the employee takes up his/her post.

The successful candidate will be recruited for an initial period of one year, with the possibility of extending the assignment.

Constraints and risks

none

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