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Reference : UMR5075-CYNDAV-004
Workplace : GRENOBLE
Date of publication : Thursday, January 31, 2019
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 25 March 2019
Proportion of work : Full time
Remuneration : 1913€ based on experience
Desired level of education : 2-year university degree
Experience required : 1 to 4 years
The recruited person will work on a project centred around the function of intrinsically disordered proteins, i.e. proteins without stable three-dimensional structure, which are implicated in endocytosis, the major cellular import pathway. In this project, the research technician will prepare recombinant proteins in E.coli end will maintain a mammalian cell culture.
- Expression and purification of recombinant proteins in E.coli
- Preparation of proteins labelled with stable isotopes for nuclear magnetic resonance
- Site-specific protein labelling with fluorophores
- Expression and purification of proteins with unnatural amino acids
- Cloning of protein constructs for expression in E.coli (and mammalian cells)
- Mammalian cell culture and transient transfection
- Organisation/Composition of protocols
- Knowledge of molecular biology and biochemistry
- Bacterial culture (E.coli)
- Affinity purification
- Size exclusion chromatography
- Cell culture and transfections
- Rigour and precision
- Ability to work in a team and show initiative
- French would be a plus
The Institut de Biologie Structurale (IBS, http://www.ibs.fr/spip.php?lang=en) is situated on the EPN science campus in Grenoble, in proximity to the ESRF (European Synchrotron Radiation Facility), the EMBL (European Molecular Biology Laboratory) and the ILL (Institute Laue-Langevin).
The research technician will work in the ERC team of Sigrid Milles at the IBS in Grenoble. The team is investigating the function of the intrinsically disordered proteins contained in the endocytic pathway by nuclear magnetic resonance (NMR) and single molecule fluorescence. The IBS provides access to an NMR facility (hosting 3 x 600, 700, 850 and 950 MHz spectrometers) and the single molecule fluorescence equipment is developed within the team.
Facilities for molecular biology, biochemistry and cell biology are available and the IBS shares access to various research platforms via the ISBG (Integrated Structural Biology Grenoble, http://www.isbg.fr/spip.php?lang=en)
12-month renewable contract
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